APPLIED PLANT BIOTECHNOLOGY

Abstract

Plant tissue culture is a practice used to propagate plants under sterile conditions, often toproduce clones of a plant. Different techniques in plant tissue culture offer advantages overtraditional methods of propagation including the production of exact copies of plants even inabsence of seeds and low germinating seeds, production of genetically modified and disease free plants. This handout entitled plant tissue culture course is a subject aimed at 3rd year Biotechnology and 1 st year Master genetic and amelioration of plant students. The content of this handout includes the program taught in the department of natural and life sciences at Ibn Khaldoun University in Tiaret. It is written in the form of a detailed course, with illustrative diagrams. It is presented in a very simple style which allows students to understand very quickly. The content of this handout is structured in different chapters, wich we devoted the important of tissue culture diffentspractises and we have included the description of the technic of transgenesis and modification of the genom